To address whether nigericin induced-pyroptosis enhanced the anti-tumor effect of T cells with the combination of PD-1 antibody, MDA-MB-231 cells were cultured with human peripheral blood mononuclear cells (PBMCs) isolated from human healthy donors (Figure S4A), followed by treatment with nigericin, anti-PD-1 or their combination. The gene discussed is PDCD1; the disease is neoplasm.