TGFB1 and ovarian carcinoma: Interestingly, shape factor quantification of over 500 OVCAR3 cells and nuclei under TGF-β1, and hypoxia treatment on soft substrates showed a significant reduction in both cell (CSF) and nuclear shape factor (NSF) (Figure 1D,E), suggesting that synergistic chemical and mechanical cues could be sufficient to induce EMT-related morphological changes in this less invasive and more epithelial ovarian cancer cell line.