QRT-PCR and Western-blot showed that MBD3 was differentially expressed in colon cancer cell lines, among which the expression level was the highest in the SW620 cell line, the lowest in the CaCO2 cell line, and the second and third in the HCT116 and SW480 cell lines, respectively (Figure 6A). The gene discussed is MBD3; the disease is colonic neoplasm.