Studies on DIPGs by human-derived primary cell lines or iPSC harbouring H3K27M not only revealed consistent CSC markers of CD133, SOX2, Nestin, PAX6, Vimentin, and differentiation markers of MAP2, GFAP, NeuN, NOTCH 1, and CSPG4 in previous studies on high-grade gliomas, but also introduced new CSC markers of ALDH and controversial markers of Olig2 and PDGFRα; due to the origins of DIPG, CSCs differed in different research groups [16,17,18,19]. This evidence concerns the gene SOX2 and central nervous system cancer.