We subsequently analyzed a set of human CRC (n = 9) and found a dramatic decrease in the CD4−/CD8− T cells fraction (mean 61.5 cells/mm2, 3.8% of CD3+ T cells, p = 0.0011; Figure 1G,M and Supplementary Figure S1C) also in the context of CRC-associated tertiary lymphoid structures (TLS, Supplementary Figure S17) (n = 6; mean 60 cells/mm2, 2.7% of CD3+ T cells; p < 0.0001; Figure 1G). Here, CD4 is linked to colorectal carcinoma.