GTF2IRD1 and Werner syndrome: To determine the role Gtf2ird1 plays in the WS behavioral repertoire, we first generated and validated a novel mouse overexpressing the general transcription factor GTF2IRD1 (TG‐Gtf2ird1‐HA) via its endogenous regulatory elements, engineered using a BAC with an HA tag that was inserted just prior to a stop codon of Gtf2ird1 (Figure 1A).