Gene silencing of ACCα, elongation of very long chain fatty acid-like 1 (ELOVL1), FASN, insulin-induced gene 1 (INSIG1), sterol regulatory element-binding protein cleavage-activating protein (SCAP), stearoyl-CoA desaturase (SCD), and thyroid hormone-responsive protein (THRSP) reduced the lipidomic profiles and viability of the BC cells. The gene discussed is ACACA; the disease is breast cancer.