In this study, we demonstrated the beneficial effects of formononetin on AP and associated intestinal injury, and mechanistically formononetin reduces ROS-mediated inflammation response through Kelch Like ECH Associated Protein 1 (Keap1)- Nuclear factor erythroid2-related factor 2 (Nrf2) signaling pathway, ultimately inhibits intestinal barrier dysfunction and bacterial translocation to alleviate AP. Here, KEAP1 is linked to alkaline phosphatase measurement.