Maternal age, serum HCG level, EMT on HCG day, the number of pregnancies, types of transferred embryos, basal E2, basal PRL, LH level on HCG day, AMH, infertility duration, method of fertilizations, E2 level on HCG day, the number of left sinus follicles, stimulation protocols, endometrial preparation protocol, and Gn were identified as the predictive factors after the first cycle. The gene discussed is PLOD1; the disease is Infertility.