ROCK1 and hepatocellular carcinoma: Treatment with GL-V9 in the absence of proliferation inhibitory concentrations in MHCC-97H and HCC-LM3 cells (Fig. 3D and Supplementary Fig. S4A, B), significantly reduced the migration and invasion (Fig. 4A–C), and the phosphorylation of AMPK, MLC and ROCK1 protein (Fig. 4D).