Treatment with GL-V9 in the absence of proliferation inhibitory concentrations in MHCC-97H and HCC-LM3 cells (Fig. 3D and Supplementary Fig. S4A, B), significantly reduced the migration and invasion (Fig. 4A–C), and the phosphorylation of AMPK, MLC and ROCK1 protein (Fig. 4D). The gene discussed is PRKAA2; the disease is hepatocellular carcinoma.