The objectives of this prospective clinical trial are to determine the growth parameters during the NICU admission in preterm infants with GA≤33 weeks and birth weight less than 1500 g (VLBW) and evaluate the effect of additional enteral protein supplement on the anthropometric parameters after reaching full enteral feed with either FBM or PF; we also aimed to check the effect of high protein milk on albumin, BUN levels and venous blood gas analysis, and incidence of NEC. This evidence concerns the gene ALB and necrotizing enterocolitis.