This study used PCR-DNA sequencing and real-time PCR to examine potential immune (TLR4, TLR7, TNF-α, IL10, NCF4, and LITAF), antioxidant (ATOX1, GST, and OXSR1), and erythritol-related (TKT, RPIA, and AMPD1) gene efficacy as candidates for prediction and tracking endometritis resistance/susceptibility in postparturient Holstein dairy cows. This evidence concerns the gene TLR4 and endometritis.