The study by Li et al. [26] reported a 20-fold increase in Tc17 cell frequency of healthy and colorectal cancer patient naïve CD8+ T-cell cultures upon 7-day culture in IMDM media using anti-CD3/CD28 mAb stimulation with IL-2, IL-1β, IL-6, IL-23, TGFβ, anti-IL-4, and anti-IFNγ. This evidence concerns the gene CD28 and colorectal cancer.