To understand the age-group difference in B cells underlying a successful influenza vaccination response, we profiled the B cells enriched from PBMC samples prior to receiving vaccination (day 0, D0) and 7 days post-vaccination (D7) from three young and three older subjects using 10x Genomics paired single-cell RNAseq and BCR sequencing (Figure 1A). The gene discussed is BCR; the disease is influenza.