3-(4,5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT), O staining and the TG and TC content-determination assays proved that the FFA-induced NAFLD model of HepG2 cells is suitable for studying the activity and mechanisms of MSP1–MSP18 (Figure 2 and Figure 3A). Here, ATAD1 is linked to metabolic dysfunction-associated steatotic liver disease.