The effects of MSP1–MSP18 on lipid accumulation in the NAFLD cell model were investigated, and MSP2, MSP8, MSP10, MSP13 and MSP18 presented significant lipid lowering effects, demonstrating the significantly ameliorating functions of MSP2, MSP8, MSP10, MSP13 and MSP18 on FFA-induced HepG2 cells (Figure 5, Figure 6 and Figure 7). This evidence concerns the gene ATAD1 and metabolic dysfunction-associated steatotic liver disease.