NES and Alzheimer disease: On the other hand, the iPSC-NSC AD cell line (CW50018) shows a notable difference in the protein expression levels of Nestin (p < 0.0001), in addition to the differences in the expression levels of MSI1(p = 0.0001) and Sox1 (p < 0.0066) when cultured on Matrigel vs. the PL matrix.