AKT1 and glioblastoma: We then confirmed that SC79 could rescue GBM invasion decline caused by SLC25A32 knockdown by Transwell assay, 3D sphere invasion assay and ex vivo co-culture invasion model (Fig. 7A, B, D), indicating that SLC25A32 also increased GBM invasion through the PI3K-AKT-mTOR signaling pathway.