To dissect molecular pathways involved in RCC progression following 4q9p21 loss, we generated genetically engineered kidney organoids (GEKOs) carrying somatic knockouts of Nf2 and Setd2 TSGs along with the inactivation of Cdkn2a/b on chromosome 4q9p21 and performed single-cell RNA-sequencing (scRNA-seq) analysis, aiming to provide a dynamic multi-dimensional landscape of 9p deletion in RCC evolution (Fig. 3a and Extended Data Fig. 6a). This evidence concerns the gene SETD2 and renal cell adenocarcinoma.