The results showed that the levels of P-PKCθ in the liver were increased in KO NAFLD mice compared to those in WT NAFLD mice (Fig. 9A, B, P < 0.001), while the expression of P-PKCθ in the liver was significantly decreased in MYDGF-replenished mice compared to AAV-GFP mice (Fig. 9A, B, P < 0.001). The gene discussed is PRRT2; the disease is metabolic dysfunction-associated steatotic liver disease.