Importantly, the mRNA expression of the lipid synthesis genes SREBP1c, acetyl-CoA carboxylase 1 (ACC1), fatty acid synthase (FAS), and stearoyl-CoA desaturase 1 (SCD1) in isolated hepatocytes was significantly elevated in KO NAFLD mice compared to WT NAFLD mice; however, the mRNA expression of the lipid oxidation genes carnitine palmitoyltransferase 1a (CPT1a), acyl-coenzyme A oxidase 1 (ACOX1), and peroxisome proliferator-activated receptor alpha (PPARα) was not significantly different between KO NAFLD mice and WT NAFLD mice (Fig. 1D). This evidence concerns the gene ACOX1 and metabolic dysfunction-associated steatotic liver disease.