Our results suggested that P-IKKβ expression in the liver was increased in KO NAFLD mice compared to WT NAFLD mice (Fig. 5D, P < 0.001), but was significantly decreased in MYDGF-replenished mice compared to AAV-GFP mice (Fig. 5E, P < 0.001). This evidence concerns the gene IKBKB and metabolic dysfunction-associated steatotic liver disease.