The results showed that the levels of P-PKCθ in the liver were increased in KO NAFLD mice compared to those in WT NAFLD mice (Fig. 9A, B, P < 0.001), while the expression of P-PKCθ in the liver was significantly decreased in MYDGF-replenished mice compared to AAV-GFP mice (Fig. 9A, B, P < 0.001). Here, MYDGF is linked to metabolic dysfunction-associated steatotic liver disease.