In light of the recent research associating a truncated variant of ASTL with infertility (Maddirevula et al., 2022), and the observation of altered fertility in ASTL−/− and FETUB−/− female mice (Burkart et al., 2012; Dietzel et al., 2013), we conducted an analysis of nsSNPs and regulatory SNPs in ASTL from the dbSNP database. This evidence concerns the gene FETUB and Infertility.