By comparing the epigenetic changes associated with Nrf2 induction in the 5-FU-resistant colon cancer cell line SNUC5, it was concluded that Nrf2 expression, as well as its nuclear translocation and promoter binding, were markedly elevated in SNUC5/5-FUR cells compared to SNUC5 cells, and further Nrf2 or HO-1 knockdown mediated by siRNA considerably curtailed the proliferation of colon cancer cells both in vitro and in vivo, leading to heightened sensitivity to 5-FU (150). This evidence concerns the gene NFE2L2 and malignant colon neoplasm.