Some of the most upregulated genes found in CRC co-cultured macrophages – LRP5, DPYSL3, THBS1, CXCL8, IL1ß and EREG (Figure 1E) – are related to the Wnt pathway (42–46), which is constitutively activated in CRC (47). Here, DPYSL3 is linked to colorectal carcinoma.