We confirmed that cellular O-GlcNAcylation levels and O-GlcNAcylation of RIPK1 were attenuated by TSZ treatment, as was the case when necroptosis was induced in erythrocytes by in vivo LPS-induced endotoxemia (Figures 3A, S3A). This evidence concerns the gene RIPK1 and serum lipopolysaccharide activity.