To validate at the transcriptome level that the engineered destabilized 3’UTR of ERBB2 degraded ERBB2 and its interactome through the designed mechanism incorporated in the ERBB2 3’UTR-destabilizing constructs, we performed RNA sequencing on the ERBB2-expressing EGFR T790M lung cancer cells NCI-H1975 WT, NCI-H1975 vector, and in NCI-H1975 cancer cells containing desARE3’UTR ERBB2-3 and desARE3’UTR ERBB2-30. This evidence concerns the gene EGFR and lung cancer.