Using a mouse model of peritoneal fibrosis induced by PD, we confirmed that injection of TGF‐β‐EVs aggravates PD‐induced peritoneal fibrosis, while impairing EVs secretion by Rab27a knockdown leads to the alleviation of peritoneal fibrosis and improvement of peritoneal dysfunction. The gene discussed is TGFB1; the disease is Peritoneal Fibrosis.