Using a mouse model of peritoneal fibrosis induced by PD, we confirmed that injection of TGF‐β‐EVs aggravates PD‐induced peritoneal fibrosis, while impairing EVs secretion by Rab27a knockdown leads to the alleviation of peritoneal fibrosis and improvement of peritoneal dysfunction. Here, RAB27A is linked to Peritoneal Fibrosis.