ENP patients had olfactory dysfunction and tissue eosinophilia versus NENP patients. IL25, IL33 and TSLP levels in ENP patients were higher versus NENP and controlsAA stimulated IL33 and TSLP production but not IL25 for 24 and 48 h. IL25 was not increased by allergensAA enhanced IL33 and TSLP and IL6 but AF only increased IL6 production. AA enhanced phosphorylated NF‐κB and c‐Jun expression, but AF did not. IL33 and TSLP expression was regulated by NF‐κB, AP‐1 and MAPK. Here, TSLP is linked to atrial fibrillation.