AF extract supressed Poly I:C activated IRF‐3 but not NF‐κB. AF extract also triggered rapid dephosphorylation of IFNβ induced phospho‐STAT1Suppressive effect of AF on induction of CXCL10 by TLR3 activators was mediated via activation of PAR2 and in part by PTPN11 activationPAR2 activation mobilised phosphatases that reduced TLR3 signalling through STAT and IRF. Here, TRIM63 is linked to atrial fibrillation.