Knock‐down of RAGE and TLR4 receptors as well as calprotectin proteins significantly inhibited IL25 and TSLPCalprotectin was involved in AA‐induced TSLP and IL25 production as well as the ATP pathway and played an important role in Th2 airway inflammationAirborne allergens stimulated production and release of calprotectin and protease activity was crucial in this processAllergen induced calprotectin was increased in cells from CRS patients. The gene discussed is TSLP; the disease is congenital rubella syndrome.