In this study, we evaluated a panel of 8 biomarkers, including B7-H3, IL-6, PLA2G7, Tie-2, GDF-15, IL-6 R alpha, sSDC1, and VCAM-1 selected based on their reported relevancy to ovarian cancer and multiplexing feasibility as a customized magnetic bead-based 8-plex immunoassay. The gene discussed is CD276; the disease is ovarian cancer.