Cells were cotransfected with a plasmid encoding Spike and a bicistronic plasmid encoding ORF8-Strep-IRES-eGFP and harvested and incubated with sera collected from three COVID-19 convalescent (Fig. 7, A and B) (Table S1A) or three COVID-19 negative (Fig. 7A) human donors. Here, CHMP5 is linked to COVID-19.