MAPT and supranuclear palsy, progressive, 1: To address these perplexing aspects of disease pathogenesis, this study used RNAscope combined with immunostaining, complemented by single-nucleus (sn)RNA sequencing from PSP and control human brains, to systematically map and quantify MAPT expression in neurons, oligodendrocytes and astrocytes across different brain regions to determine whether there are cell type and/or regional differences in MAPT expression.