Because KRAS, TP53, and PTEN mutants are the major drivers of intrahepatic cholangiocarcinoma (ICC) [43], we used LbCas12a-N57 to simultaneously insert KrasG12D into the Rosa26 site and disrupt Trp53 and Pten in the mouse liver. This evidence concerns the gene TP53 and intrahepatic cholangiocarcinoma.