To confirm the synergy of PEV plus TOP1 inhibitors in CRC cells, we tested combinations of PEV with SN38, the active metabolite of irinotecan, by performing the ATPlite luminescence assay to measure cell viability in 6 other cultured CRC cell lines including 4 MSI (Microsatellite Instability)-positive CRC lines SW48, SW837, KM12 and HCT15 (Fig. 1e) and 2 MSI-negative and CIN (chromosome instability)-positive CRC lines HT29 and SW620. The gene discussed is TOP1; the disease is colorectal carcinoma.