ABHD4 and obesity due to melanocortin 4 receptor deficiency: Overall, because 1) there is a positive correlation between adipose tissue Abhd4 gene expression and adiposity/obesity in mice (Fig. 1B–D), and 2) Abhd4 mRNA was abundant in both SV cell and adipocyte fractions of adipose tissue (Fig. 1E, F) as well as in both 3T3-L1 pre-adipocytes and newly differentiated mature adipocytes (Fig. 1G), we chose a loss-of-function approach instead of a gain-of-function approach to better understand the functional role of Abhd4 in adipocyte differentiation and lipid biology.