The expression level of Klotho protein is also related to aging, and Kuro-o found that mice lacking Klotho suffer from premature aging syndrome.[23] Previous evidence has shown that Klotho deficiency and CKD cause aging, CVD, and bone disease.[24] Furthermore, there is a strong association between aging and the incidence of several age-related chronic diseases, such as diabetes and inflammatory bowel disease.[25,26] Thus, an unexpected discovery was made that apoB may be related to aging. This evidence concerns the gene APOB and diabetes mellitus.