The protein expression trend was consistent with that of mRNA, and semiquantitative analysis showed that HSF1 expression in subjects with high-risk PC(Gleason score ≥ 8) was significantly higher than that noted in subjects with medium-risk (Gleason score = 7) and low-risk PC (Gleason score = 6) (Fig. 2D). The gene discussed is HSF1; the disease is pachyonychia congenita.