Immunohistochemistry analysis revealed a significant increase in endothelial iNOS expression in the iNOS-transfected endothelial cells (Figure 3A, lower middle and right panels, yellow overlap), while Sham-transfected tumor endothelial cells had no change in iNOS compared with untreated tumors (Figure 3A, middle row shows no increase in iNOS, green, compared to untreated controls). This evidence concerns the gene NOS2 and neoplasm.