To verify the reasonable grouping of the exploration set and validation set, statistical analysis was performed on the two parts using Wilcoxon Rank Sum Test for HER2-IHC, HER2-FISH, HER2/CEP17 ratio, HER2-SRM expression level, and TNM staging, and using χ2 test for FISH results, gender, Lauren classification, lymph node metastasis, differentiation degree, and tumor location. This evidence concerns the gene ERBB2 and metastatic malignant neoplasm in the lymph nodes.