Then, the clinical prognostic data of COAD patients were acquired from TCGA, and subsequently, 152 diff-ARGs and 125 diff-ERGs were combined with the clinical data using univariate Cox analysis (P<0.05), to eventually obtain 12 prognosis-related ARGs (TIMP1, BDNF, IGF1, CDKN2A, MTA1, NAT1, INHBB, CD24, CD36, TRAF2, NOTCH3, PPP2R2A) and 11 prognosis-related ERGs (BGN, CXCL1, FSTL3, GPC1, MMP3, OXTR, PCOLCE2, SCG2, SERPINE1, SERPINH11, TPM2) (Figure 2A, B). This evidence concerns the gene NAT1 and colon adenocarcinoma.