Furthermore, no difference in EdU+ cell percentage and wound closure ratio were observed after treatment of β-Estradiol (10nM) and testosterone (100nM) in si-Scrambled and si-Gpr68 group (Figures 3E–G) Taken together, these results exclude the possibility that the gender-dependent differential effect on melanoma growth is due to direct regulation of GPR68 by sex hormones. The gene discussed is GPR68; the disease is melanoma.