It has previously been shown that TcdB causes internalization of NHE3 in cell cultures.18 The authors hypothesized that glucosylation of Rho GTPases by TcdB could lead to disruption of ERM and NHERF proteins, leading to internalization of NHE3.18 We examined the expression of pERM and NHERF1 during infection at 2 dpi and found no quantifiable differences. The gene discussed is NHERF1; the disease is infection.