Interestingly, volcano plot revealed that compared with the WT group, 11-Dodecenoic acid (11-DA) in liver was significantly down-regulated (p < 0.05 and |log2FC|≥ 1) in the Plin5 KO group under HFHC diet conditions (Fig. 6B, C), suggesting that 11-DA may be an important metabolite downstream of Plin5 during NASH progression. This evidence concerns the gene PLIN5 and metabolic dysfunction-associated steatohepatitis.