IP followed by WB assays showed that the O-GlcNAcylation on p65, and the interaction between p65 and Ogt was significantly decreased in AD mice, but the interaction between p65 and Gsk3β was significantly increased in the hippocampus of AD mice compared to Ctrl mice (Additional file 9: Fig. S9j, IP part, Additional file 9: Fig. S9o–t). Here, GSK3B is linked to Alzheimer disease.