Multiple variables were proved to be significantly associated with the PFS of NSCLC patients with ICI treatment, including treatment lines(p = 0.0097), smoking(p = 0.0040), PD-L1 expression(p = 0.0112), TMB(p = 0.0380) and 20-gene mutation signature(p = 0.0030) (Fig. 5A-E). Here, CD274 is linked to non-small cell lung carcinoma.