EPCAM and non-small cell lung carcinoma: Herein, extending beyond our previous work on CTCs and circulating tumor endothelial cells(CTECs) in NSCLC [11, 12], the EpCAM-independent subtraction enrichment and immunostaining-fluorescence in situ hybridization (SE-iFISH) platform was optimized to enrich and perform comprehensive in situ morphologic, karyotypic and phenotypic characterization of a full spectrum of aneuploid CTCs and CTC-WBC clusters in advanced NSCLC receiving first-line treatment.