Although the frequency of PD-1+ cells was comparable both in total CD8+ and CD4+ Tconv and Treg cells (Supplementary Fig. 3B), as well as in CD4+ and CD8+ T cell subsets (Supplementary Fig. 3C), tumor-infiltrating terminally differentiated EMRA CD4+ T cells were among the subsets with significantly higher PD-1 expression, at the mean fluorescence intensity (MFI) level, in PF vs PD (Fig. 4D). Here, CD4 is linked to neoplasm.