TNF and infection: At 24 h post-infection, significantly less TNF was present in supernatants of Psme1/2-/- BMDMs infected with L. pneumophila ΔflaA or ΔΔ (pEV) compared to WT BMDMs but no differences in secretion from BMDMs infected with L. pneumophila ΔΔ (plegC4) (S2B Fig).