AFP and liver cancer: The transcriptomic cluster #2 of SC08 did not express AFP but highly expressed GPC3 and multiple genes that were highly expressed in normal liver and liver cancer, such as apolipoprotein genes (APOA1, APOA2 and APOB), transferrin gene (TF), fibrinogen genes (FGG and FGA) and coagulation factor II gene (F2), and the sampling regions of cluster #2 (PT1, PT2 and PT5) were classified as HAS according to the histopathological features.