CD40 and cancer: To test the effect CSF1Ri and agonistic anti-CD40 mAb treatment of MTS on the secretion of inflammatory/anti-inflammatory cytokines, we measured TNF-α and IL-10 proteins in the co-culture conditioned medium at 7 days, a timepoint where we observed a major reduction in cancer cell proliferation and viability (Figures 4D, 5B).