To evaluate the effect of iron deficiency on cell proliferation of germ cells, primary spermatocyte and Sertoli cells in testis and epididymis, we tested the co-staining of DDX4+/Ki67+, SCP3+/Ki67+ GATA1+/Ki67+ and GATA1+/BrdU+. Here, MKI67 is linked to Iron deficiency anemia.