Based on gene expression profiling and CUT&TAG sequencing of knock‐in human cell lines, we identified several potential common and specific pathways, that is, JAK‐STAT, NOTCH signalling pathway and B‐cell development, and genes, that is, CPNE7, VWDE, LTK and IGSF9B, of IKZF1 N159S‐positive AML. The gene discussed is LTK; the disease is acute myeloid leukemia.