Flow cytometry of annexin V/PI staining was performed to evaluate the effects of various IKZF1 mutants on apoptosis of K562 and U937 leukaemia cells, which suggested that WT IKAROS and G158S could induce more apoptosis as compared with N159S and N159Y (Figure 3C and D). The gene discussed is ANXA5; the disease is leukemia.