Lastly, A375 displayed the most advanced stage of melanoma development by clear melanoma expansion into the reconstructed dermis and by the secretion of a plethora of (anti-)inflammatory cytokines (GM-CSF, IL-6, IL-8, IL-10, M-CSF, and TGFβ) involved in immune suppression and melanoma progression. The gene discussed is TGFB1; the disease is melanoma.