The presence of the A375 melanoma cells in the skin model led to an increased release of Flt-1 (2-fold increase), PlGF (2-fold increase), Tie-2 (1.8-fold increase), VEGF (1.8-fold increase), VEGF-C (1.7-fold increase), and VEGF-D (1.9-fold increase) (Figure 5a). This evidence concerns the gene TEK and melanoma.